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High-throughput screening degli enzimi carboidrati di degradazione Utilizzando Novel insolubile Chromogenic Substrate Assay Kit
JoVE 신문
생화학
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JoVE 신문 생화학
High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
DOI:

06:51 min

September 20, 2016

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Chapters

  • 00:05Title
  • 00:40Chromogenic Assay with CPH Substrates, Detection, and Quantification
  • 02:41Chromogenic Assay with Insoluble Chromogenic Biomass Substrates, Detection, and Quantification
  • 04:15Results: Enzymatic Degradation of Chromogenic Polymer Hydrogel Substrates
  • 06:02Conclusion

Summary

자동 번역

A high-throughput assay for enzyme screening is described. This multiplexed ready-to-use assay kit comprises of pre-chosen Chromogenic Polymer Hydrogel (CPH) substrates and complex Insoluble Chromogenic Biomass (ICB) substrates. Target enzymes are polysaccharide degrading endo-enzymes and proteases.

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