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JoVE Journal Biology
Working with Auditory HEI-OC1 Cells
 

Working with Auditory HEI-OC1 Cells

Article DOI: 10.3791/54425-v • 08:56 min • September 3rd, 2016
September 3rd, 2016 •
1, 1, 1, 1
1Head and Neck Surgery, David Geffen School of Medicine, University of California, Los Angeles

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요약
Automatic Translation

House Ear Institute-Organ of Corti 1 (HEI-OC1) is one of the few mouse auditory cell lines currently available for research purposes. This protocol describes how to work with HEI-OC1 cells to investigate the cytotoxic effects of pharmacological drugs as well as functional properties of inner ear proteins.

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Keywords: HEI-OC1 Cells Auditory Cells Prestin Cell Culture Cell Passaging Cell Freezing Trypsinization DMEM FBS Cell Growth
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