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Chapters
- 00:05Title
- 00:58Modifying a Polystyrene Surface for Printing
- 02:29Etching Patterns into the Polystyrene Sheet
- 05:48Preparing and Printing Cell-containing Gelatin Bioink into the Etched Patterns
- 08:50Results: Constraint of Printed Stem Cells
- 09:29Conclusion
This protocol describes a bioprinting methodology using an automated robotic depositing system that incorporates etched topographical guidance cues with the precision deposition of a cell bearing hydrogel bioink. The printed cells are directly delivered to the etched features and are able to sense and orientate with them.
Tags
Keywords:
Automated Robotic DispensingSurface GuidanceBioprintingCell BehaviorCell DistributionTopographical Cell Guidance2D Cell PatternsBack Pressure-assisted Robotic DispensingSurface EtchingExtrusion-based BioprintingPolystyrene SheetOxygen Plasma TreatmentFetal Bovine SerumCell DeliveryProgrammed Pattern
