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1Dept. of Clinical and Experimental Medicine,University of Pisa, 2OtoLab,Azienda Ospedaliero-Universitaria Pisana (AOUP), 3Dept. of Civil and Industrial Engineering,University of Pisa, 4Immunohematology Operative Unit,Azienda Ospedaliero-Universitaria Pisana (AOUP), 5Dept. Of Surgical, Medical, Molecular Pathology and Emergency Medicine,University of Pisa, 6II Orthopedic and Traumatologic Clinic,Azienda Ospedaliero-Universitaria Pisana (AOUP)
Chapters
- 00:05Title
- 00:57Preparation of Autologous Serum
- 02:20Isolation of hMSCs from Bone Marrow
- 03:42Culture of hMSCs in the Presence of Autologous Serum
- 05:19Osteogenic Pre-induction and Recovery of hMSCs
- 06:20Preparation of hMSCs/Fibrin Clot Constructs and Cell Viability Assay
- 07:30Results: hMSCs/Fibrin Clot Generation and Application
- 09:10Conclusion
Coltura umane cellule mesenchimali stromali (hMSCs) con siero autologo, riduce i rischi di rigetto di materiale xenogenico e altri effetti negativi. Esso permette inoltre il recupero di un sottoinsieme di progenitori mesodermici, che può trasportare hMSCs freschi. Incorporare hMSCs in un coagulo di fibrina autologa consente una facile maneggevolezza ed efficace impianto chirurgico.
