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JoVE Journal Developmental Biology
Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity
 

Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity

Article DOI: 10.3791/54934-v • 08:30 min • February 24th, 2017
February 24th, 2017 •
1, 1, 1
1CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Division of Rheumatology, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea

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요약
Automatic Translation

Mechanical stress can induce the chondrogenic differentiation of stem cells, providing a potential therapeutic approach for the repair of impaired cartilage. We present a protocol to induce the chondrogenic differentiation of adipose-derived stem cells (ASCs) using centrifugal gravity (CG). CG-induced upregulation of SOX9 results in the development of chondrogenic phenotypes.

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Keywords: Chondrogenic Differentiation Adipose-derived Stem Cells Centrifugal Gravity Cartilage Repair Prechondrogenic Differentiation Osteogenic Differentiation Fibrogenic Differentiation Cytokines EDTA Hemocytometer Micromass Culture Chondrogenic Differentiation Medium TGF-beta-1
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