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Journal / Bioengineering / A Microfluidic Flow Chamber Model for Platelet…
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Bioengineering

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JoVE Journal Bioengineering
A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
 

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Article DOI: 10.3791/55351-v • 09:38 min • February 14th, 2017
February 14th, 2017 •
1,2, 1, 1, 2,3,4, 1, 1,2,3
1Transfusion Research Center, Belgian Red Cross-Flanders, 2Faculty of Medicine and Health Sciences, Ghent University, 3Blood Service, Belgian Red Cross-Flanders, 4Department of Public Health and Primary Care, KULeuven - University of Leuven

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요약
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This paper describes an experimental model of hemostasis that simultaneously measures platelet function and coagulation. Platelet and fibrin fluorescence is measured in real-time, and platelet adhesion rate, coagulation rate, and onset of coagulation are determined. The model is used to determine platelet procoagulant properties under flow in concentrates for transfusion.

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Microfluidic Flow Chamber Platelet Transfusion Hemostasis Platelet Deposition Fibrin Formation Real-time Video Microscopy Transfusion Medicine Platelet Concentrate Preparation Procoagulant Nature Platelet Function Coagulation Contact Pathway Extrinsic Pathway Lipidated Tissue Factor Microfluidic Biochip Collagen Incubation Y-shaped Channels Mixing Bioc
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