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Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
 

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Article DOI: 10.3791/55486-v • 08:32 min • March 16th, 2017
March 16th, 2017 •
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1Institute of Molecular Biology and Biochemistry, Medical University of Graz

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This manuscript presents protocols for the application of novel genetically encoded nitric oxide (NO•) probes (geNOps) to monitor single cell NO• fluctuations in real-time using fluorescence microscopy. The Ca2+-triggered NO• formation on the level of individual endothelial cells was visualized by combining geNOps with a chemical Ca2+ sensor.

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Genetically Encoded Fluorescent Nitric Oxide Probes GeNOps Real-time Imaging Single Cells Nitric Oxide Signals Fluorescent Protein-based Probes Fluorescence Quench Kinetics Spatial Resolution Temporal Resolution Conventional Fluorescence Microscope Iron(II) Booster Solution Endothelial Cell Line HEK293 Cells Iron Loading Procedure
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