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配体激活的G蛋白偶联受体内在化的检测通过共聚焦显微镜
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JoVE 신문 생물학
Detection of Ligand-activated G Protein-coupled Receptor Internalization by Confocal Microscopy
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配体激活的G蛋白偶联受体内在化的检测通过共聚焦显微镜

DOI:
10.3791/55514-v

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10:24 min

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April 09, 2017

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, , , , ,
1National Engineering Research Center of Marine Facilities Aquaculture, Marine Science College,Zhejiang Ocean University, 2Institute of Biochemistry, College of Life Sciences,Zhejiang University

Chapters

  • 00:05Title
  • 00:49Cell Seeding
  • 02:05Cell Transfection Method 1 with Liposome Formulations
  • 03:44Cell Culture on Coverslips
  • 04:37Cellular Localization Using Confocal Microscopy
  • 05:47Cellular Internalization
  • 06:42Confocal Microscopy
  • 07:43Results: Gonadotropin Releasing Hormone Receptor Internalization Detection
  • 09:21Conclusion

Summary

자동 번역

자동 번역

这个协议描述了在哺乳动物细胞中的G蛋白偶联受体（GPCR）的内化共聚焦显微镜检测。它包括基本细胞培养，转染，和共聚焦显微镜的程序和提供了一个高效和容易解释的方法来检测融合表达GPCR的亚细胞定位和内化。

Tags

Keywords: GPCRLigand-activatedInternalizationConfocal MicroscopySubcellular LocalizationReceptor RecyclingHEK293 CellsTransfectionPlasmid DNAReduced Serum MediumReagentDMEMFBS

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