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Developmental Biology

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JoVE Journal Developmental Biology
En 5-mC prikkeblot-analyse, der kvantificerer DNA-methyleringsniveauet for chondrocyt-differentiering
 
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En 5-mC prikkeblot-analyse, der kvantificerer DNA-methyleringsniveauet for chondrocyt-differentiering

Article DOI: 10.3791/55565-v • 10:07 min • May 17th, 2017
May 17th, 2017 •
1,2, 3, 4,5, 2,6, 2, 2, 1,2
1Guangzhou Medical University, 2Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), 3Department of Chemistry, The Chinese University of Hong Kong, 4School of Biomedical Engineering, Shanghai Jiao Tong University, 5Renji Hospital Clinical Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine, 6Shantou University Medical College

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Vi præsenterer en metode til kvantificering af DNA-methylering baseret på 5-methylcytosin (5-mC) dot blot. Vi fastslog 5-mC niveauerne under chondrocyt dedifferentiering. Denne enkle teknik kunne bruges til hurtigt at bestemme chondrocytfænotypen ved ACI-behandling.

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Udviklingsbiologi udgave 123 cellekultur monolags ekspansion chondrocyt dedifferentiering DNA-methylering 5-mC dot blot-analyse genomisk DNA-ekstraktion
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