Journal
/
생물학
/
A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research

A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research

DOI:
10.3791/55768-v

•

09:35 min

•

August 16, 2017

•

, , , , ,
1Department of Experimental Toxicology and ZEBET,German Federal Institute for Risk Assessment (BfR), 2Department of Clinical Pharmacology and Toxicology,Charité-Universitätsmedizin Berlin

Chapters

  • 00:05Title
  • 00:54Performing the Game Set Enrichment Analysis (GSEA)
  • 05:46Comparing the GSEA Results and Identifying the Optimal Animal Model
  • 07:55Results: Overlap of Pathway Regulation Identifies Suitable and Unsuitable Animal Models
  • 08:49Conclusion

Summary

자동 번역

자동 번역

We provide a standardized protocol for the use of gene set enrichment analysis of transcriptomic data to identify an ideal mouse model for translational research.
This protocol can be used with DNA microarray and RNA sequencing data and can further be extended to other omics data if data are available.

Tags

Keywords: Gene Set Enrichment AnalysisAnimal ModelTranslational ResearchGenome DataMouse ModelsInflammatory DiseasesGene Expression StudiesGene Expression DataPhenotype FileMolecular Signature DatabaseDNA Chip AnnotationsGSEA SoftwareGene SymbolsPermutations

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/55768?article_type=v

Testing Visual Sensitivity to the Speed and Direction of Motion in Lizards
check_url/kr/55768?article_type=v

Computer-Generated Animal Model Stimuli
check_url/kr/55768?article_type=v

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
check_url/kr/55768?article_type=v

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets
check_url/kr/55768?article_type=v

The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System
check_url/kr/55768?article_type=v

Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
check_url/kr/55768?article_type=v

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
check_url/kr/55768?article_type=v

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
check_url/kr/55768?article_type=v

Xenopus laevis as a Model to Identify Translation Impairment
check_url/kr/55768?article_type=v

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

Read Article