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JoVE Journal Neuroscience
Quadruple Immunostaining af Olfactory Bulb til Visualisering af Olfactory Sensory Axon Molecular Identity Codes
 
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Quadruple Immunostaining af Olfactory Bulb til Visualisering af Olfactory Sensory Axon Molecular Identity Codes

Article DOI: 10.3791/55893-v • 06:32 min • June 5th, 2017
June 5th, 2017 •
1, 1,2, 1,3
1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 2Center for Information and Neural Networks, National Institute of Information and Communications Technology, 3PRESTO, Japan Science and Technology Agency (JST)

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Olfaktoriske sensoriske neuroner udtrykker et bredt udvalg af axon-sorteringsmolekyler for at etablere et passende neuralt kredsløb. Denne protokol beskriver en immunhistokemisk farvningsfremgangsmåde til visualisering af kombinatoriske udtryk for axon-sorteringsmolekyler ved axon termini af olfaktoriske sensoriske neuroner.

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Neurovidenskab udgave 124 olfaktorisk system immunhistokemi genekspression axon vejledning neurale kredsløbsdannelse olfaktoriske sensoriske neuroner
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