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Screening af bioaktive nanopartikler i phagocytiske immunceller til inhibitorer af Toll-lignende Receptor Signalering
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JoVE Journal Medicine
Screening Bioactive Nanoparticles in Phagocytic Immune Cells for Inhibitors of Toll-like Receptor Signaling

Screening af bioaktive nanopartikler i phagocytiske immunceller til inhibitorer af Toll-lignende Receptor Signalering

DOI:
10.3791/56075-v

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09:51 min

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July 26, 2017

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, , , ,
1Department of Respiratory Medicine, Shanghai First People’s Hospital,Shanghai Jiaotong University School of Medicine, 2Department of Pediatrics,BC Children’s Hospital and University of British Columbia

Chapters

  • 00:05Title
  • 00:57Preparation of Cell Culture Media and Reagents
  • 02:10Culture of THP-1 Reporter Cell-derived Macrophages
  • 03:45Screening for Potential TLR4 Nano-inhibitors Using the Reprter Cells
  • 07:22Results: Inhibition of Toll-like Receptor Signaling
  • 09:13Conclusion

Summary

Automatic Translation

Automatic Translation

Toll-lignende receptor (TLR) signalering spiller en vigtig rolle i patofysiologien af ​​mange humane inflammatoriske sygdomme, og regulering af TLR-responser af bioaktive nanopartikler forventes at være gavnlig under mange inflammatoriske tilstande. THP-1-cellebaserede reporterceller tilvejebringer en alsidig og robust screeningsplatform til identifikation af nye inhibitorer af TLR-signalering.

Tags

Bioactive NanoparticlesToll-like Receptor SignalingInnate Immune CellsInflammatory DiseasesPhagocytic Immune CellsReporter Cell SystemsNanoinhibitorsScreening ProcedureLuciferase SubstrateSEAP SubstrateCell CulturePMA

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