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筛选生物活性纳米颗粒在噬菌体免疫细胞中的Toll样受体信号传导抑制剂

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JoVE Journal Medicine

Screening Bioactive Nanoparticles in Phagocytic Immune Cells for Inhibitors of Toll-like Receptor Signaling

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筛选生物活性纳米颗粒在噬菌体免疫细胞中的Toll样受体信号传导抑制剂

DOI:

10.3791/56075-v

•

09:51 min

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July 26, 2017

•

Hong Yang, Shan Yu Fung, Aihua Bao, Qiang Li, Stuart E. Turvey

¹Department of Respiratory Medicine, Shanghai First People’s Hospital,Shanghai Jiaotong University School of Medicine, ²Department of Pediatrics,BC Children’s Hospital and University of British Columbia

Chapters

00:05Title
00:57Preparation of Cell Culture Media and Reagents
02:10Culture of THP-1 Reporter Cell-derived Macrophages
03:45Screening for Potential TLR4 Nano-inhibitors Using the Reprter Cells
07:22Results: Inhibition of Toll-like Receptor Signaling
09:13Conclusion

Summary

Automatic Translation

Toll样受体（TLR）信号在许多人类炎性疾病的病理生理学中起着重要作用，并且预期在许多炎症状态下调节生物活性纳米颗粒的TLR应答是有益的。基于THP-1细胞的报道细胞提供了一个通用和强大的筛选平台，用于鉴定TLR信号传导的新型抑制剂。

Tags

Keywords: Bioactive Nanoparticles Toll-like Receptor Signaling Innate Immune Cells Inflammatory Diseases Phagocytic Immune Cells Reporter Cell Systems Nanoinhibitors Screening Procedure Luciferase Substrate SEAP Substrate Cell Culture PMA

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