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JoVE Journal Neuroscience
使用快速、简单且便宜的 AWESAM 协议培养在体内像小鼠星形胶质细胞
 
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使用快速、简单且便宜的 AWESAM 协议培养在体内像小鼠星形胶质细胞

Article DOI: 10.3791/56092-v • 07:56 min • January 10th, 2018
January 10th, 2018 •
1,2,3, 3
1Chemical Biology, Chemistry Research Laboratory, University of Oxford, 2Department of Physiology, Anatomy, and Genetics, University of Oxford, 3Trans-synaptic signaling, European Neuroscience Institute

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这里描述的 AWESAM 协议是最理想的培养小鼠星形胶质细胞与其他脑神经干细胞的快速, 简单, 便宜的方式。AWESAM 星形胶质细胞展示自发 Ca2 +信号, 形态学和基因表达谱类似于星形胶质细胞在体内。

Tags

神经生物学 问题 131 星形胶质细胞 单养 皮质 海马 老鼠 大鼠 钙 囊泡 突 中等
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