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JoVE Journal Bioengineering
CRISPR/Cas9 复合原注射液生产基因工程金黄叙利亚仓鼠
 
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CRISPR/Cas9 复合原注射液生产基因工程金黄叙利亚仓鼠

Article DOI: 10.3791/56263-v • 06:12 min • January 9th, 2018
January 9th, 2018 •
*1, *1,2, 1, 3, 3,4, 2,5, 1
1Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 2National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences, Zhengzhou University, 3Department of Animal Science Division of Applied Life Science (BK21 Plus), Gyeongsang National University, 4Institute of Agriculture and Life Science, Gyeongsang National University, 5Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London
* These authors contributed equally

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原 (PN) 注射的聚类定期 interspaced 短复发重复 (CRISPR) 和 CRISPR 相关 protein-9 核酸 (CRISPR/Cas9) 系统是一种高效的方法生产基因工程金黄叙利亚仓鼠。在此, 我们描述了详细的 PN 注射协议生产的基因剔除仓鼠与 CRISPR/Cas9 系统。

Tags

生物工程 问题 131 原注射液 CRISPR/Cas9 基因编辑 淘汰赛 受精卵 胚胎移植 动物模型 金黄叙利亚仓鼠
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