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Journal / Genetics / Immunfärgning för DNA ändringar: tillämpad analys …
JoVE Journal
Genetics

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JoVE Journal Genetics
Immunfärgning för DNA ändringar
 
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Immunfärgning för DNA ändringar: tillämpad analys av Confocal bilder

Article DOI: 10.3791/56318-v • 09:42 min • September 7th, 2017
September 7th, 2017 •
*1, *1, 1, 1, 1, 2, 2, 1, 3, 1
1Division of Cancer and Stem Cells, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, 2School of Life Sciences Imaging (SLIM), School of Life Sciences, University of Nottingham, 3Children's Brain Tumour Research Centre, School of Medicine, QMC, University of Nottingham
* These authors contributed equally

챕터

요약
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Nyupptäckt oxiderat former av 5-metylcytosin (oxi-mCs), 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) och 5-carboxylcytosine (5caC) kan representera olika DNA ändringar med unika funktionella roller. Här beskrivs ett semikvantitativt arbetsflöde för visualisering av oxi-mCs' rumsliga fördelning, signal intensiteten profilering och colocalization.

Tags

Problemet 127 DNA epigenetik genetik oxi-mCs N6-methyladenine immunohistokemi Zen konfokalmikroskopi Signal kvantitering rumsliga fördelningen
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