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Journal / Genetics / Immunostaining des Modifications de l’ADN :…
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Genetics

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JoVE Journal Genetics
Immunostaining des Modifications de l’ADN 
 
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Immunostaining des Modifications de l’ADN : Computational Analysis of Images confocales

Article DOI: 10.3791/56318-v • 09:42 min • September 7th, 2017
September 7th, 2017 •
*1, *1, 1, 1, 1, 2, 2, 1, 3, 1
1Division of Cancer and Stem Cells, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, 2School of Life Sciences Imaging (SLIM), School of Life Sciences, University of Nottingham, 3Children's Brain Tumour Research Centre, School of Medicine, QMC, University of Nottingham
* These authors contributed equally

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Nouvellement découvert des formes oxydées de la 5-méthylcytosine (oxi-mCs), 5-hydroxyméthylcytosine (5hmC), 5-formylcytosine (5FC) et 5-carboxylcytosine (5caC) peut-être représenter des modifications d’ADN distinctes avec des rôles fonctionnels uniques. Un workflow semi quantitatif pour la visualisation de la répartition spatiale des oxi-mCs, profilage d’intensité de signal et colocalisation est décrit ici.

Tags

Numéro 127 épigénétique ADN génétique oxi-mCs N6-méthyladénine immunohistochimie Zen microscopie confocale quantification du Signal Distribution spatiale
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