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JoVE Journal Chemistry
A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
 

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

Article DOI: 10.3791/56445-v • 13:35 min • March 1st, 2018
March 1st, 2018 •
1, 3, 4, 1, 2
1School of Public Health of Southeast University, Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou, 2Key Laboratory of Child Development and Learning Science (Ministry of Education), School of Biological Science & Medical Engineering, Southeast University, 3School of Public Health, Tianjin Medical University, 4British Columbia Academy, Nanjing Foreign Language School

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요약
Automatic Translation

We present a convenient solid-phase extraction coupled to high-pressure liquid chromatography (HPLC) with electrochemical detection (ECD) for simultaneous determination of three monoamine neurotransmitters and two of their metabolites in infants' urine. We also identify the metabolite MHPG as a potential biomarker for the early diagnosis of brain damage for infants.

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Keywords: High-pressure Liquid Chromatography Catecholamine Neurotransmitters Metabolites Solid-phase Extraction Electrochemical Detection Monoamine Neurotransmitters Infant's Urine DPBA Solution Norepinephrine Epinephrine Dopamine MHPG DOPAC DHBA Hydrochloric Acid Analyte Stocks
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