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Journal / Developmental Biology / Kvantitativ hele-mount Immunofluorescence analyse …
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Developmental Biology

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JoVE Journal Developmental Biology
Kvantitativ hele-mount Immunofluorescence analyse av Cardiac stamfar populasjoner i Mouse embryoer
 
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Kvantitativ hele-mount Immunofluorescence analyse av Cardiac stamfar populasjoner i Mouse embryoer

Article DOI: 10.3791/56446-v • 09:42 min • October 12th, 2017
October 12th, 2017 •
1,2,3, 4, 5,6, 1,2,3
1Cell, Developmental, and Regenerative Biology Department, Icahn School of Medicine at Mount Sinai, 2Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, 3Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, 4Microscopy Core, Icahn School of Medicine at Mount Sinai, 5Department of Neuroscience, Icahn School of Medicine at Mount Sinai, 6Friedman Brain Institute, Icahn School of Medicine at Mount Sinai

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Her beskriver vi en protokoll for hele mount immunofluorescence og bildebasert kvantitative volumetric analyse av tidlig stadium mouse embryoer. Vi presenterer denne teknikken som en kraftig tilnærming til kvalitativt og kvantitativt vurdere cardiac strukturer under utvikling, og foreslår at det kan være mye tilpasses andre organsystemer.

Tags

Utviklingspsykologi biologi problemet 128 AC Confocal mikroskopi bildeanalyser kvantitative bildebehandling organogenesen heart utvikling musen embryo
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