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Developmental Biology

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JoVE Journal Developmental Biology
Kvantitativa hela-mount immunofluorescens analys av hjärt stamceller populationer i musembryon
 
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Kvantitativa hela-mount immunofluorescens analys av hjärt stamceller populationer i musembryon

Article DOI: 10.3791/56446-v • 09:42 min • October 12th, 2017
October 12th, 2017 •
1,2,3, 4, 5,6, 1,2,3
1Cell, Developmental, and Regenerative Biology Department, Icahn School of Medicine at Mount Sinai, 2Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, 3Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, 4Microscopy Core, Icahn School of Medicine at Mount Sinai, 5Department of Neuroscience, Icahn School of Medicine at Mount Sinai, 6Friedman Brain Institute, Icahn School of Medicine at Mount Sinai

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Här beskriver vi ett protokoll för hela berget immunofluorescens och image-baserad kvantitativ volymetrisk analys av tidiga skede musembryon. Vi presenterar denna teknik som en kraftfull metod att kvalitativt och kvantitativt bedöma hjärt strukturer under utveckling, och föreslår att det kan vara allmänt anpassas till andra organsystem.

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Utvecklingsbiologi fråga 128 konfokalmikroskopi bildanalys kvantitativa imaging organogenes hjärtat utveckling mus embryo
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