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Journal / Biology / 无透镜视频显微术在黏附细胞培养动态定量分析中的研究…
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无透镜视频显微术在黏附细胞培养动态定量分析中的研究
 
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无透镜视频显微术在黏附细胞培养动态定量分析中的研究

Article DOI: 10.3791/56580-v • 09:05 min • February 23rd, 2018
February 23rd, 2018 •
1, 1, 2, 2, 3,4, 3, 1, 1, 1, 5, 5, 4,6, 1
1CEA, LETI, DTBS, LISA, Université Grenoble Alpes, 2CEA, LETI, DTBS, LBAM, Université Grenoble Alpes, 3CEA, INSERM, BIG, Université Grenoble Alpes, 4CNRS, FR CNRS 3425, 5CNRS, UMR 144, Molecular Mechanisms of Intracellular Transport, PSL Research University, Institut Curie, 6TIMC-IMAG

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无透镜的视频显微镜使我们能够在孵化器内直接监测细胞培养。在这里, 我们描述了用于获取和分析2.7 天长时间获取培养的 HeLa 细胞的完整协议, 导致一个数据集 2.2 x 106测量单个细胞形态学和10584细胞周期轨道。

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细胞生物学 问题 132 无透镜显微镜 视频细胞术 密集细胞培养 定量显微学
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