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Bioengineering

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JoVE Journal Bioengineering
Detección visual de los ácidos nucleicos múltiples en un arreglo de capilares
 
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Detección visual de los ácidos nucleicos múltiples en un arreglo de capilares

Article DOI: 10.3791/56597-v • 08:56 min • November 15th, 2017
November 15th, 2017 •
*1,2,3, *1,2,3, *4, 4, 1,2,3, 4,5, 1,2,3, 6, 6, 4, 1,2,3
1Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 2State Key Laboratory of Oncogenes and Related Genes, 3School of Biomedical Engineering, Shanghai Jiao Tong University, 4Collaborative Innovation Center for Biosafety of GMOs, National Center for the Molecular Characterization of Genetically Modified Organisms, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 5Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, 6Department of Biomedical Engineering, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, Tsinghua University
* These authors contributed equally

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Este protocolo describe la fabricación de una cinta pequeña, lista para usar que se puede aplicar para detección visual de varios ácidos nucleicos en una prueba individual, que es fácil de operar. En este enfoque, una gama capilar fue utilizada para la detección multiplex y altamente eficiente de los objetivos de la OMG.

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Detección de ácido nucleico multiplex de bioingeniería número 129 capilar matriz lámpara organismos modificados genéticamente con (OMG) detección visual fácil de operar
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