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Journal / Biochemistry / 高通量, 用定量斑点杂交分析法绝对测定组织层中选定蛋白质的含量…
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Biochemistry

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JoVE Journal Biochemistry
高通量, 用定量斑点杂交分析法绝对测定组织层中选定蛋白质的含量 (QDB)
 
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高通量, 用定量斑点杂交分析法绝对测定组织层中选定蛋白质的含量 (QDB)

Article DOI: 10.3791/56885-v • 08:10 min • August 21st, 2018
August 21st, 2018 •
*1, *2, 1, 3, 2, 1, 1,4, 2,3, 1
1Medicine and Pharmacy Research Center, Binzhou Medical University, 2Yantai Zestern Biotechnique Co. LTD, 3Precision Medicine research center, Binzhou Medical University, 4Department of Chemistry - BMC, Uppsala University
* These authors contributed equally

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在这里, 我们展示了定量斑点杂交分析 (QDB) 的详细过程, 通过确定目标蛋白的绝对含量, 帽肌动蛋白, gelsolin 样 (CAPG), 在三种不同的小鼠组织。我们展示了高通量, 方便, 定量应用免疫印迹技术的生物标志物验证在细胞和组织水平。

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生物化学 138 期 定量斑点杂交分析 (QDB) 应用免疫印迹 高通量 绝对测定 Elisa 分析。
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