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Journal / Genetics / 整合酶缺陷慢病毒载体载体的制备及其在细胞 CRISPR/Cas9-mediated…
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JoVE Journal Genetics
整合酶缺陷慢病毒载体载体的制备及其在细胞 CRISPR/Cas9-mediated 基因敲除中的表达
 
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整合酶缺陷慢病毒载体载体的制备及其在细胞 CRISPR/Cas9-mediated 基因敲除中的表达

Article DOI: 10.3791/56915-v • 10:42 min • December 12th, 2017
December 12th, 2017 •
1, 1
1Duke Viral Vector Core, Department of Neurobiology, Duke University School of Medicine

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我们描述了整合酶缺乏慢病毒载体载体 (IDLVs) 作为向细胞传递 CRISPR/Cas9 的工具的生产策略。由于能够在细胞中调节快速和健壮的基因编辑, IDLVs 为基因的传递提供了一个更安全、同样有效的载体平台, 与整合酶能力的载体相比。

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遗传学 问题 130 病毒介导的基因转移 HEK-293T 细胞 整合酶缺乏慢病毒载体载体 CRISPR/Cas9 基因编辑系统, p 24 ELISA 蔗糖梯度离心 转移酶
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