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Journal / Neuroscience / 激光捕获显微切割分离新鲜冷冻人体肠神经节的优化…
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JoVE Journal Neuroscience
激光捕获显微切割分离新鲜冷冻人体肠神经节的优化
 
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激光捕获显微切割分离新鲜冷冻人体肠神经节的优化

Article DOI: 10.3791/57762-v • 08:28 min • June 14th, 2018
June 14th, 2018 •
1, 1, 1
1Vanderbilt University Medical Center

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该协议的目的是获得高完整性的 RNA 样本从无固定的, 新鲜切除的人肠道组织, 利用激光捕获显微切割 (LCM) 分离。该协议包括制备人体肠道组织、cryosectioning、乙醇染色和脱水、LCM 和 RNA 提取的闪光冷冻样品。

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神经科学 问题 136 激光捕获显微切割 (LCM) 人肠道组织 肠道 肠神经节 肠道神经系统 rna 提取 甲酚紫染色 rna 序列 (rna 排序)
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