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Immunology and Infection

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JoVE Journal Immunology and Infection
Label-gratis identifikation af lymfocyt undertyper ved hjælp af tre-dimensionelle kvantitative fase Imaging og Machine Learning
 
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Label-gratis identifikation af lymfocyt undertyper ved hjælp af tre-dimensionelle kvantitative fase Imaging og Machine Learning

Article DOI: 10.3791/58305-v • 08:58 min • November 19th, 2018
November 19th, 2018 •
1, 2,3,4,7, 3,4,5, 2,3, 6, 4, 2,3, 2,3,4
1Department of Physics, University of Cambridge, 2Department of Physics, Korea Advanced Institute of Science and Technology, 3KAIST Institute for Health Science and Technology, Korea Advanced Institute of Science and Technology, 4Tomocube, Inc., 5Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 6Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 7Department of Applied Physics, Stanford University

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Vi beskriver en protokol for etiket-fri identifikation af lymfocyt undertyper ved hjælp af kvantitative fase billeddiagnostik og en maskine læring algoritme. Målinger af 3D brydningsindeks Tomogrammer af lymfocytter præsenterer 3D morfologiske og biokemiske oplysninger for individuelle celler, som er derefter analyseret med en maskine-læring algoritme for identifikation af celletyper.

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Immunologi og infektion sag 141 kvantitative fase imaging optisk diffraktion tomografi holotomography holografisk mikroskopi lymfocyt identifikation immun celle immunologi maskinen læring etiket-gratis billedbehandling
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