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Journal / Immunology and Infection / 用三维定量相位成像和机器学习技术对淋巴细胞亚型进行无标签鉴定…
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Immunology and Infection

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JoVE Journal Immunology and Infection
用三维定量相位成像和机器学习技术对淋巴细胞亚型进行无标签鉴定
 
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用三维定量相位成像和机器学习技术对淋巴细胞亚型进行无标签鉴定

Article DOI: 10.3791/58305-v • 08:58 min • November 19th, 2018
November 19th, 2018 •
1, 2,3,4,7, 3,4,5, 2,3, 6, 4, 2,3, 2,3,4
1Department of Physics, University of Cambridge, 2Department of Physics, Korea Advanced Institute of Science and Technology, 3KAIST Institute for Health Science and Technology, Korea Advanced Institute of Science and Technology, 4Tomocube, Inc., 5Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 6Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 7Department of Applied Physics, Stanford University

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我们描述了一种使用定量相位成像和机器学习算法对淋巴细胞亚型进行无标签识别的协议。对淋巴细胞三维折射率图的测量为单个细胞提供了三维形态和生化信息, 然后用机器学习算法进行分析, 以识别细胞类型。

Tags

免疫学与感染 第14期 定量相位成像 光学衍射断层扫描 全息成像 全息显微镜 淋巴细胞鉴定 免疫细胞 免疫学 机器学习 无标签成像
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