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JoVE Journal Neuroscience
Gleichzeitige Kryoschneiden mehrere Nagetier Gehirne
 
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Gleichzeitige Kryoschneiden mehrere Nagetier Gehirne

Article DOI: 10.3791/58513-v • 06:37 min • September 18th, 2018
September 18th, 2018 •
1,2,3, 1, 5, 1,3,4, 1,3,4
1Department of Child Health, University of Arizona College of Medicine, 2Department of Biological Sciences, University of Bath, 3BARROW Neurological Institute at Phoenix Children's Hospital, 4Phoenix Veteran Affairs Healthcare System, 5Department of Basic Medical Sciences, University of Arizona College of Medicine

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Hier präsentieren wir ein Protokoll, um einzufrieren und Abschnitt Gehirngewebe von mehreren Tieren als eine zeitsparende Alternative zur Bearbeitung einzelner Gehirne. Dies senkt die Färbung Variabilität bei Immunohistochemistry und reduziert Zeit Kryoschneiden und Imaging.

Tags

Neurowissenschaften Ausgabe 139 Neurowissenschaften Histologie Immunhistochemie Nagetier Cryoprotection Kryoschneiden Kryostat Neuropathologie
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