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1Laboratory of New Techniques of Restoration & Reconstruction, Institute of Traumatology & Orthopedics,Nanjing University of Chinese Medicine, 2TCM Nursing Intervention Laboratory of Chronic Disease Key Laboratory,Nanjing University of Chinese Medicine, 3Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing,Nanjing University of Chinese Medicine, 4Department of Traumatology and Orthopedics,Affiliated Hospital of Nanjing University of Chinese Medicine
Chapters
- 00:04TItle
- 00:55Traditional Method of Isolating Bone Marrow-derived Cells
- 02:40Improved Method of Isolating Bone Marrow-derived Cells
- 04:11Purification by Density Gradient Centrifugation
- 05:39Culture and Differentiation
- 06:15Bone Resorption Assay Using Toluidine Blue Staining
- 08:03Scanning Electron Microscopy
- 09:03Results: Analysis of the Osteoclast Precursors Isolated, Purified, and Differentiated from Rat Bone Marrow
- 10:40Conclusion
Osteoclasts are tissue-specific macrophage polykaryons derived from the monocyte-macrophage lineage of hematopoietic stem cells. This protocol describes how to isolate bone marrow cells so that large quantities of osteoclasts are obtained while reducing the risk of accidents found in traditional methods.
