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Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow
 

Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow

Article DOI: 10.3791/58895-v • 11:11 min • May 19th, 2019
May 19th, 2019 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 3, 3, 1,2,4
1Laboratory of New Techniques of Restoration & Reconstruction, Institute of Traumatology & Orthopedics, Nanjing University of Chinese Medicine, 2TCM Nursing Intervention Laboratory of Chronic Disease Key Laboratory, Nanjing University of Chinese Medicine, 3Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, 4Department of Traumatology and Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine

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Summary
Automatic Translation

Osteoclasts are tissue-specific macrophage polykaryons derived from the monocyte-macrophage lineage of hematopoietic stem cells. This protocol describes how to isolate bone marrow cells so that large quantities of osteoclasts are obtained while reducing the risk of accidents found in traditional methods.

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Keywords: Osteoclast Precursors Bone Marrow Isolation Purification Differentiation Cell Culture Cell Extraction Cell Counting Osteoporosis Bone Metabolism
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