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Immunology and Infection

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JoVE Journal Immunology and Infection
Skræddersy in vivo cytotoksiske assays til at studere immun dominans i tumor specifikke CD8+ T- celle respons
 
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Skræddersy in vivo cytotoksiske assays til at studere immun dominans i tumor specifikke CD8+ T- celle respons

Article DOI: 10.3791/59531-v • 10:13 min • May 6th, 2019
May 6th, 2019 •
1, 1, 1,2,3,4
1Department of Microbiology and Immunology, Western University, 2Department of Medicine, Division of Clinical Immunology and Allergy, Western University, 3Department of Surgery, Division of General Surgery, Western University, 4Lawson Health Research Institute

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Vi beskriver her en flow cytometri-baseret in vivo drab assay, der muliggør undersøgelse af immun dominans i cytotoksiske T-lymfocyt (CTL) svar til en model tumor antigen. Vi giver eksempler på, hvordan denne elegante analyse kan anvendes til Mekanistiske undersøgelser og til Drug effektivitet test.

Tags

Immunologi og infektion CD8+ T celler cytotoksicitet kræft immun dominans tumor-afledte peptider store t-antigen regulatoriske t-celler PD-1 kontrolpunkts-hæmmere Drug effekt test flow cytometri
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