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Immunology and Infection

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JoVE Journal Immunology and Infection
Maatwerk in vivo chemo tests om Immunodominance in tumor-specifieke CD8+ T Cell responsen te bestuderen
 
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Maatwerk in vivo chemo tests om Immunodominance in tumor-specifieke CD8+ T Cell responsen te bestuderen

Article DOI: 10.3791/59531-v • 10:13 min • May 6th, 2019
May 6th, 2019 •
1, 1, 1,2,3,4
1Department of Microbiology and Immunology, Western University, 2Department of Medicine, Division of Clinical Immunology and Allergy, Western University, 3Department of Surgery, Division of General Surgery, Western University, 4Lawson Health Research Institute

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We beschrijven hier een flow Cytometry-based in vivo doden assay die het mogelijk maakt onderzoek van immunodominance in chemo T lymfocyten (CTL) Reacties op een model tumor antigeen. Wij bieden voorbeelden van hoe deze elegante assay kan worden gebruikt voor mechanistische studies en voor de effectiviteit van geneesmiddelen testen.

Tags

Immunologie en infectie CD8+ T cellen chemo- kanker immunodominance tumor-afgeleide peptiden grote T-antigeen regulerende t-cellen PD-1 Checkpoint-remmers drug effectiviteit testen flow Cytometry
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