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Biochemistry

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JoVE Journal Biochemistry
Expression und Reinigung von Nuklease-freier Sauerstofffänger Protocatechuate 3,4-Dioxygenase
 
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Expression und Reinigung von Nuklease-freier Sauerstofffänger Protocatechuate 3,4-Dioxygenase

Article DOI: 10.3791/59599-v • 10:14 min • November 8th, 2019
November 8th, 2019 •
1, 1, 1, 1
1Cancer Biology and Genetics, College of Medicine, The Ohio State University

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Protocatechuate 3,4-Dioxygenase (PCD) kann mit seinem Substrat Protocatechuic Säure (PCA) enzymatisch freien diatomischen Sauerstoff aus einem wässrigen System entfernen. Dieses Protokoll beschreibt die Expressions-, Reinigungs- und Aktivitätsanalyse dieses Sauerstoff-Aufräumenzyms.

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Biochemie Ausgabe 153 Chromatographie Protocatechuat-3,4-Dioxygenase (PCD) Nukleasekontamination Protocatechuinsäure (PCA) reaktive Sauerstoffspezies Sauerstoffaufräumsysteme (OSS)
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