Isolatie en cultuur van primaire aorta-endotheel cellen van miniatuur varkens

Article DOI: 10.3791/59673-v • 06:38 min • August 14th, 2019

August 14th, 2019 •

Yanli Zhao^1,2,3, Chengjiang Zhao⁴, David K.C. Cooper⁵, Ying Lu², Kewang Luo⁶, Huiyun Wang³, Pengfei Chen³, Changchun Zeng³, Shaodong Luan¹, Lisha Mou², Hanchao Gao^1,2,3

¹Department of Nephrology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, ²Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, ³Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, ⁴Department of Endocrinology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, ⁵Xenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, ⁶People's Hospital of Longhua

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Er wordt een effectieve enzymatische methode voor de isolatie van primaire varkens aorta endotheel cellen (pAECs) beschreven. De geïsoleerde primaire pAECs kan worden gebruikt om de immuunrespons en coagulatie reactie bij xenotransplantatie te onderzoeken.

Isolatie en cultuur van primaire aorta-endotheel cellen van miniatuur varkens

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