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JoVE Journal Genetics
In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster In Vivo
 
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In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster In Vivo

Article DOI: 10.3791/59720-v • 10:00 min • July 11th, 2019
July 11th, 2019 •
1, 1, 1, 1, 1, 2, 2, 1, 2, 1
1Department of Biological Sciences, University of Alabama, 2Department of Genetics, University of Wisconsin-Madison

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Nous présentons un protocole utilisant une approche génétique avancée au dépiste pour des mutants présentant la neurodégénérescence dans le melanogaster de Drosophila. Il intègre un test d'escalade, l'analyse histologique, la cartographie des gènes et le séquençage de l'ADN pour finalement identifier de nouveaux gènes liés au processus de neuroprotection.

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Génétique Numéro 149 Drosophila melanogaster neurodégénérescence dépistage génétique avancé analyse d'escalade histologie cartographie génétique séquençage de l'ADN
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