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Chapters
- 00:05Title
- 01:01Preparation for Aniline Tagging
- 02:41Labeling Reaction and LC/MS Procedure
- 06:06Results: Identification and Labeling Results of Metabolites
- 07:02Conclusion
Here, we present a robust protocol to quantify 40 compounds involved in central carbon and energy metabolism in cell-free protein synthesis reactions. The cell-free synthesis mixture is derivatized with aniline for effective separation using reversed-phase liquid chromatography and then quantified by mass spectrometry using isotopically labelled internal standards.
