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JoVE Journal Cancer Research
Isolatie van stam-achtige cellen uit 3-dimensionale Spheroid-culturen
 
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Isolatie van stam-achtige cellen uit 3-dimensionale Spheroid-culturen

Article DOI: 10.3791/60357-v • 09:06 min • December 13th, 2019
December 13th, 2019 •
1, 1, 1, 1, 1,2,3
1Department of Urology, College of Medicine, University of Illinois at Chicago, 2Department of Pathology, College of Medicine, University of Illinois at Chicago, 3University of Illinois Cancer Center, College of Medicine, University of Illinois at Chicago

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Met behulp van humane primaire prostaat Epitheelcellen, rapporteren we een nieuwe biomarker-vrije methode van functionele karakterisering van stam-achtige cellen door een spheroid-gebaseerde label-retentie test. Een stap-voor-stap protocol wordt beschreven voor BrdU, CFSE of far Red 2D Cell labeling; driedimensionale spheroid-formatie; Label-behoud stam-achtige celidentificatie door immunocytochemie; en isolatie door FACS.

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Kankeronderzoek probleem 154 stamcellen voorlopercellen celisolatie spheroids prostaat kanker preparaten
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