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Journal / Immunology and Infection / 使用IBA1和TMEM119进行小鼠脑微胶质密度、形态学和周围骨髓细胞渗透分析的免疫荧光染色…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
使用IBA1和TMEM119进行小鼠脑微胶质密度、形态学和周围骨髓细胞渗透分析的免疫荧光染色
 
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使用IBA1和TMEM119进行小鼠脑微胶质密度、形态学和周围骨髓细胞渗透分析的免疫荧光染色

Article DOI: 10.3791/60510-v • 10:40 min • October 27th, 2019
October 27th, 2019 •
1,2, 1,2, 1,3, 1,2,4, 1,2,4, 1,2
1Axe Neurosciences, Centre de Recherche du CHU de Québec-Université Laval, 2Département de Médecine Moléculaire, Faculté de Médecine, Université Laval, 3Integrated Program in Neuroscience, McGill University, 4Center for Brain Immunology and Glia (BIG), University of Virginia

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该协议描述了IBA1和TMEM119免疫荧光结化的分步工作流程，除了分析微胶质密度、分布和形态，以及小鼠脑组织中外周髓细胞渗透。

Tags

免疫学和感染， 问题 152， 小鼠， 大脑， 微胶质， 骨髓细胞， 神经免疫学， 免疫荧光， IBA1， TMEM119， 显微镜
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