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Murine Precision-Cut Liver Slices comme un modèle Ex Vivo de biologie du foie
 
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Murine Precision-Cut Liver Slices comme un modèle Ex Vivo de biologie du foie

Article DOI: 10.3791/60992-v • 12:36 min • March 14th, 2020
March 14th, 2020 •
*1, *1, 2,3, 1,4,5, 6, 7, 8,9, 2,10, 1,4
1Hepatic Fibrosis Group, QIMR Berghofer Medical Research Institute, 2School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, 3School of Veterinary and Life Sciences, Murdoch University, 4School of Medicine, The University of Queensland, 5Madrid Institute for Advanced Studies (IMDEA) in Food, CEI UAM+CSIC, 6School of Medicine, Griffith University, 7School of Biological Sciences, Queen's University Belfast, 8Department of Gastroenterology & Hepatology, Fiona Stanley and Fremantle Hospital Group, 9School of Medical and Health Sciences, Edith Cowan University, 10Centre for Cell Therapy and Regenerative Medicine, School of Biomedical Sciences, University of Western Australia
* These authors contributed equally

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Ce protocole fournit une méthode simple et fiable pour la production de tranches de foie de précision viables de souris. Les échantillons de tissu ex vivo peuvent être maintenus dans des conditions de culture tissulaire de laboratoire pendant plusieurs jours, fournissant un modèle flexible pour examiner la pathobiologie de foie.

Tags

Médecine Numéro 157 foie tranches vibratome ex vivo hépatocytes tissus culture cellules stellates
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