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Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
 

Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay

Article DOI: 10.3791/61122-v 06:19 min May 12th, 2020
May 12th, 2020

챕터

요약

The goal of the thiobarbituric acid reactive substances assay is to assess oxidative stress in biological samples by measuring the production of lipid peroxidation products, primarily malondialdehyde, using visible wavelength spectrophotometry at 532 nm. The method described here can be applied to human serum, cell lysates, and low density lipoproteins.

Tags

Keywords: TBARS Assay Oxidative Stress Biological Samples MDA Thiobarbituric Acid Sodium Hydroxide Sodium Acetate Spectrophotometric Analysis Sample Preparation Standard Curve
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