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Journal / Developmental Biology / Isolation of Endocardial and Coronary Endothelial …
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart
 

Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart

Article DOI: 10.3791/61126-v • 08:22 min • April 15th, 2020
April 15th, 2020 •
1,2,3, 1,2,3, 1,2,3,4,5, 1,2,3,4,5
1Department of Pediatrics, Division of Cardiology, Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease, Stanford School of Medicine, 3Stanford Cardiovascular Institute, Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute, Cincinnati Children's Hospital Medical Center

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요약
Automatic Translation

We present a protocol for the isolation of endocardial and coronary endothelial cells from rat hearts through sequential tissue digestion in a digestion buffer, cell collection from recurrent centrifuge cycles, and cell purification using anti-rat CD31 microbeads.

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Keywords: Endocardial Cells Coronary Endothelial Cells Heart Isolation Cell Type-specific Gene Expression Heart Diseases Transcriptomics Epigenetics Sprague Dawley Rats Ventricular Free Wall Dissection Digestion Buffer
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