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Journal / Immunology and Infection / Polarisering af M1 og M2 Human Monocyte-afledte…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Polarisering af M1 og M2 Human Monocyte-afledte celler og analyse med Flow Cytometry på Mycobacterium tuberkulose Infektion
 
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Polarisering af M1 og M2 Human Monocyte-afledte celler og analyse med Flow Cytometry på Mycobacterium tuberkulose Infektion

Article DOI: 10.3791/61807-v • 10:43 min • September 18th, 2020
September 18th, 2020 •
1,2, 1, 1, 3, 1, 1,4, 1
1Center for Infectious Medicine (CIM), Department of Medicine Huddinge, ANA Futura, Karolinska Institutet, 2Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, 3Clinical Microbiology, Department of Laboratory Medicine (Labmed), ANA Futura, Karolinska Institutet, 4Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet

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Denne protokol giver en metode til at studere Mycobacterium tuberkulose infektion i humane M1- eller M2-polariserede makrofager baseret på differentiering af perifere blod-monocytter til makrofaglignende celler, der er inficeret med GFP-mærket virulent stamme H37Rv, og analyseret med flow cytometry ved hjælp af en 10-farve panel, herunder udtryk for udvalgte M1/M2 markører.

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Denne måned i JoVE makrofag human polarisering M1/M2 Mycobacterium tuberkulose infektion immunrespons flow cytometry
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