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Chapters
- 00:05Introduction
- 00:49Protein Extraction from Brain Tissue
- 02:04Protein Quantification and Quality Check
- 02:32Protein Digestion
- 03:58Desalting and Peptide Quantification
- 05:06Transition List Preparation of Finalised Targets
- 07:30LC Parameters
- 08:03MS Parameters
- 08:25Run Sequence and Instrument QC
- 09:08Method Refinement
- 10:58Results and Conclusions
The protocol aims to introduce the use of a triple quadrupole mass spectrometer for Multiple Reaction Monitoring (MRM) of proteins from clinical samples. We have provided a systematic workflow starting from sample preparation to data analysis for clinical samples with all the necessary precautions to be taken.
