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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualisering af SARS-CoV-2 ved hjælp af Immuno RNA-fluorescens In Situ Hybridisering
 
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Visualisering af SARS-CoV-2 ved hjælp af Immuno RNA-fluorescens In Situ Hybridisering

Article DOI: 10.3791/62067-v • 05:23 min • December 23rd, 2020
December 23rd, 2020 •
1, 2, 1, 1,2, 1,2, 3,4, 3,4, 1
1Malopolska Centre of Biotechnology, Jagiellonian University, 2Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 3Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, The Medical University of Silesia in Katowice, 4Silesian Centre for Heart Diseases

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Her beskriver vi en simpel metode, der kombinerer RNA-fluorescens in situ-hybridisering (RNA-FISH) med immunfluorescens for at visualisere svær akut respiratorisk syndrom coronavirus 2 (SARS-CoV-2) RNA. Denne protokol kan øge forståelsen af de molekylære egenskaber ved SARS-CoV-2 RNA-værtsinteraktioner på et enkeltcellet niveau.

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Immunologi og infektion Problem 166 SARS-CoV-2 RNA-FISH Hybridisering kædereaktion Immunofluorescens Human Airway Epithelium (HAE) Permeabilization Konfokal mikroskopi
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