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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualisation du SARS-CoV-2 à l’aide de l’hybridation in situ par immuno-ARN-fluorescence
 
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Visualisation du SARS-CoV-2 à l’aide de l’hybridation in situ par immuno-ARN-fluorescence

Article DOI: 10.3791/62067-v • 05:23 min • December 23rd, 2020
December 23rd, 2020 •
1, 2, 1, 1,2, 1,2, 3,4, 3,4, 1
1Malopolska Centre of Biotechnology, Jagiellonian University, 2Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 3Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, The Medical University of Silesia in Katowice, 4Silesian Centre for Heart Diseases

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Ici, nous décrivons une méthode simple qui combine l’hybridation in situ de fluorescence d’ARN (ARN-POISSON) avec l’immunofluorescence pour visualiser l’ARN du coronavirus 2 (SARS-CoV-2) du syndrome respiratoire aigu sévère. Ce protocole peut améliorer la compréhension des caractéristiques moléculaires des interactions ARN-hôte du SARS-CoV-2 au niveau unicellulaire.

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Immunologie et infection Numéro 166 SARS-CoV-2 ARN-POISSON Hybridation en chaîne réaction Immunofluorescence Human Airway Epithelium (HAE) Perméabilisation Microscopie confocale
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