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JoVE Journal Behavior
Embryo Microinjectie en Knockout Mutant Identificatie van CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)
 
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Embryo Microinjectie en Knockout Mutant Identificatie van CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)

Article DOI: 10.3791/62068-v • 06:37 min • July 1st, 2021
July 1st, 2021 •
1,2, 2, 1, 1, 1, 2,3
1School of Chemical and Environmental Engineering, China University of Mining and Technology, 2State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 3Guangdong Laboratory of Lingnan Modern Agriculture, Shenzhen; Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences

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Hier wordt een protocol gepresenteerd van Helicoverpa armigera (Hübner) embryo micro-injectie en knock-out mutant identificatie gemaakt door CRISPR / Cas9 genoombewerking. Gemuteerde insecten maken verder onderzoek naar genfunctie en interactie tussen verschillende genen in vivo mogelijk.

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Gedrag Helicoverpa armigera embryo micro-injectie gen knock-outs CRISPR Cas9
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