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3D生物反应器培养大规模制备滑液间充质干细胞来源外泌体
JoVE 신문
생물학
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JoVE 신문 생물학
Large-Scale Preparation of Synovial Fluid Mesenchymal Stem Cell-Derived Exosomes by 3D Bioreactor Culture
DOI:

09:48 min

July 26, 2022

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Chapters

  • 00:05Introduction
  • 00:40Human Synovial Fluid Mesenchymal Stem Cells (SF-MSCs) Culture and Identification
  • 02:42Microcarrier Preparation and Perfusion Bioreactor
  • 04:18Exosome Identification and Safety Detection
  • 05:01Nanoparticle Tracking Analysis (NTA) and Western Blotting
  • 06:48Results: Identification and Analysis of SF-MSCs and the Exosomes
  • 08:55Conclusion

Summary

자동 번역

在这里,我们提出了一种使用3D生物反应器从滑液间充质干细胞产生大量GMP级外泌体的方案。

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