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Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
 

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Article DOI: 10.3791/62295-v • 07:54 min • March 31st, 2021
March 31st, 2021 •
1,2,3, 1, 2,3,4, 1, 5, 1,2,3, 1,2,3, 1,2,3, 1, 1, 1
1Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, 2International College, University of Chinese Academy of Sciences, 3Sino-Africa Joint Research Center, 4CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 5Center for Infectious and Parasitic Diseases Control Research, Kenya Medical Research Institute

챕터

요약
Automatic Translation

This work summarizes steps on developing different assays for SARS-CoV-2 detection using a two color ddPCR system. The steps are elaborate and notes have been included on how to improve the assays and experiment performance. These assays may be used for multiple SARS-CoV-2 RT-ddPCR applications.

Tags

Two-Step Reverse Transcription Droplet Digital PCR SARS-CoV-2 Detection SARS-CoV-2 Quantification Multiplex DdPCR Assays Two-color DdPCR System In-house Kits RT-qPCR RT-ddPCR CDNA Synthesis Reverse Transcription Master Mix RNA Sample Thermal Cycler Droplet Digital PCR Plate
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