Journal
/
면역학 및 감염병학
/
聚焦激光扫描显微镜为基础的定量分析 阿斯珀吉卢斯熏蒸 孔尼迪亚分布在全山光学清除鼠肺
JoVE 신문
면역학 및 감염병학
Author Produced
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung
Please note that all translations are automatically generated. Click here for the English version.

聚焦激光扫描显微镜为基础的定量分析 阿斯珀吉卢斯熏蒸 孔尼迪亚分布在全山光学清除鼠肺

DOI:
10.3791/62436-v

•

15:01 min

•

September 18, 2021

•

, , , , , ,
1Research Center for Molecular Mechanisms of Aging and Age-Related Diseases,Moscow Institute of Physics and Technology, 2Department of Immunology, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences, 3Institute of Biological Information Processing (IBI-7: Structural Biochemistry),Forschungszentrum Jülich

Chapters

  • 00:04Introduction
  • 01:03Aspergillus fumigatus Conidia Application & Specimen Preparation
  • 01:22Mouse Lung Lobe Optical Clearing
  • 02:23Mouse Lung Lobe Imaging with CLSM
  • 06:50Image Processing: Surface Rendering
  • 09:23Image Processing: Mask Correction
  • 12:54Conidia Quantitative Analysis
  • 13:41Representative Results
  • 14:07Conclusions

Summary

자동 번역

자동 번역

我们描述了对小鼠气道中 阿斯珀吉卢斯·福米加图斯 孔尼迪亚（3微米大小）分布进行定量分析的方法。该方法还可用于分析各种病理条件模型中气道中的微粒和纳米粒子聚集分布。

Tags

Confocal Laser Scanning MicroscopyAspergillus FumigatusConidiaOptical ClearingMouse LungQuantitative AnalysisRespiratory TractMucociliary ClearanceAlveolar MacrophagesNeutrophilsOptical ClearingBenzyl AlcoholBenzyl BenzoateConfocal MicroscopyLaserSpectral RangeDetector GainPinholePixel ResolutionZ-stack

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

小鼠肺树突状细胞的分离

活体显微镜的脾：寄生虫的流动性和血流量的定量分析

嗜肺军团菌外膜囊泡：隔离及对巨噬细胞促炎潜力分析

人肺树突状细胞：空间分布与表型鉴定支气管活检采用免疫组化和流式细胞仪

为了应对抑菌活性的实时成像人力初级中性粒细胞和单核细胞<em>一种。曲霉</em

激光内在人肺组织中的新型细菌特异探针的光学筛选

利用共焦显微镜可视化巨噬细胞胞外陷阱

人精切肺切片中物质的细胞毒性及免疫调节作用的评价

小鼠肺损伤的诱导,通过博霉素内切注射

小鼠特拉切斯刷细胞的分离与定量评价

Read Article