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Confocale laserscanning microscopie-gebaseerde kwantitatieve analyse van Aspergillus fumigatus Conidia distributie in whole-mount optisch gewiste muislong
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1Research Center for Molecular Mechanisms of Aging and Age-Related Diseases,Moscow Institute of Physics and Technology, 2Department of Immunology, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences, 3Institute of Biological Information Processing (IBI-7: Structural Biochemistry),Forschungszentrum Jülich
Chapters
- 00:04Introduction
- 01:03Aspergillus fumigatus Conidia Application & Specimen Preparation
- 01:22Mouse Lung Lobe Optical Clearing
- 02:23Mouse Lung Lobe Imaging with CLSM
- 06:50Image Processing: Surface Rendering
- 09:23Image Processing: Mask Correction
- 12:54Conidia Quantitative Analysis
- 13:41Representative Results
- 14:07Conclusions
We beschrijven de methode voor kwantitatieve analyse van de verdeling van Aspergillus fumigatus conidia (3 μm groot) in de luchtwegen van muizen. De methode kan ook worden gebruikt voor de analyse van microdeeltjes en nanodeeltjes agglomeraat distributie in de luchtwegen in verschillende pathologische conditiemodellen.
Tags
Confocal Laser Scanning MicroscopyAspergillus FumigatusConidiaOptical ClearingMouse LungQuantitative AnalysisRespiratory TractMucociliary ClearanceAlveolar MacrophagesNeutrophilsOptical ClearingBenzyl AlcoholBenzyl BenzoateConfocal MicroscopyLaserSpectral RangeDetector GainPinholePixel ResolutionZ-stack
